6C00

Solution structure of translation initiation factor 1 from Clostridium difficile

6C00 の概要

• エントリーDOI: 10.2210/pdb6c00/pdb
• NMR情報: BMRB: 27349
• 分子名称: Translation initiation factor IF-1 (1 entity in total)
• 機能のキーワード: translation initiation factor, translation
• 由来する生物種: Peptoclostridium difficile (Clostridium difficile)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9657.28

構造登録者

Zhang, Y.,Aguilar, F. (登録日: 2017-12-26, 公開日: 2019-01-16, 最終更新日: 2024-05-15)

主引用文献

Aguilar, F.,Banaei, N.,Zhang, Y.
1H,13C and15N resonance assignments and structure prediction of translation initiation factor 1 from Clostridium difficile.
Biomol.Nmr Assign., 13:91-95, 2019

PubMed Abstract: Clostridium difficile is a gram-positive, toxin-producing, anaerobic bacterium whose virulence factors and mechanisms of pathogenesis require further investigation. C. difficile infections (CDI) result in the severe and potentially fatal gastrointestinal diseases pseudomembranous colitis and toxic megacolon following extensive broad spectrum antibiotic treatment. The increasing C. difficile fatalities are a result of the bacteria's growing antibiotic resistance and consequential CDI recurrence, which led to the unmet need for new CDI treatment. Bacterial protein synthesis is an essential metabolic process and an effective target for antibacterial agents. Translation initiation factor 1 from C. difficile (Cd-IF1) is the smallest of the three initiation factors that acts to establish the 30S initiation complex to initiate translation during protein biosynthesis. Here we report the complete NMR H, C and N chemical shift assignments of Cd-IF1 as the basis for NMR structure determination and interaction studies. Secondary structure analyses have identified five β-strands and one short α-helix arranged in the sequential order β1-β2-β3-α1-β4-β5, which is supported by N-{H} heteroNOEs. The assigned chemical shifts were used to conduct structure prediction by CS-Rosetta. The predicted structure suggests that Cd-IF1 adopts the typical β-barrel structure and is composed of an oligomer-binding motif.

PubMed: 30370502
DOI: 10.1007/s12104-018-9858-8

実験手法

SOLUTION NMR