6BY7

Folding DNA into a lipid-conjugated nano-barrel for controlled reconstitution of membrane proteins

これはPDB形式変換不可エントリーです。

6BY7 の概要

• エントリーDOI: 10.2210/pdb6by7/pdb
• EMDBエントリー: 7304
• 分子名称: DNA (52-MER), DNA (5'-D(*TP*AP*TP*TP*AP*GP*CP*GP*AP*GP*AP*TP*GP*GP*TP*TP*TP*TP*AP*TP*TP*AP*CP*A)-3'), DNA (4346-MER), ... (118 entities in total)
• 機能のキーワード: dna nano-barrel dna origami dna assembly, dna
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 118
• 化学式量合計: 2715216.26

構造登録者

Dong, Y.,Chen, S.,Zhang, S.,Sodroski, J.,Yang, Z.,Liu, D.,Mao, Y. (登録日: 2017-12-20, 公開日: 2018-02-28, 最終更新日: 2024-03-13)

主引用文献

Dong, Y.,Chen, S.,Zhang, S.,Sodroski, J.,Yang, Z.,Liu, D.,Mao, Y.
Folding DNA into a Lipid-Conjugated Nanobarrel for Controlled Reconstitution of Membrane Proteins.
Angew. Chem. Int. Ed. Engl., 57:2072-2076, 2018

PubMed Abstract: Building upon DNA origami technology, we introduce a method to reconstitute a single membrane protein into a self-assembled DNA nanobarrel that scaffolds a nanodisc-like lipid environment. Compared with the membrane-scaffolding-protein nanodisc technique, our approach gives rise to defined stoichiometry, controlled sizes, as well as enhanced stability and homogeneity in membrane protein reconstitution. We further demonstrate potential applications of the DNA nanobarrels in the structural analysis of membrane proteins.

PubMed: 29266648
DOI: 10.1002/anie.201710147

実験手法

ELECTRON MICROSCOPY (7.5 Å)