6BU9

Drosophila Dicer-2 bound to blunt dsRNA

6BU9 の概要

• エントリーDOI: 10.2210/pdb6bu9/pdb
• EMDBエントリー: 7290 7291
• 分子名称: Dicer-2, isoform A, RNA (5'-R(*GP*GP*AP*GP*GP*UP*AP*GP*UP*AP*GP*GP*UP*UP*GP*UP*AP*UP*AP*GP*UP*AP*GP*U)-3'), RNA (5'-R(*AP*CP*UP*AP*CP*UP*AP*UP*AP*CP*AP*AP*CP*CP*UP*AP*C)-3') (3 entities in total)
• 機能のキーワード: dicer, dcr2, dcr-2, dmdcr-2, dicer-2, helicase, dsrna, rna, rna binding protein, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Drosophila melanogaster (Fruit fly); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 231384.59

構造登録者

Shen, P.S.,Sinha, N.K.,Bass, B.L. (登録日: 2017-12-09, 公開日: 2017-12-27, 最終更新日: 2024-03-13)

主引用文献

Sinha, N.K.,Iwasa, J.,Shen, P.S.,Bass, B.L.
Dicer uses distinct modules for recognizing dsRNA termini.
Science, 359:329-334, 2018

PubMed Abstract: Invertebrates rely on Dicer to cleave viral double-stranded RNA (dsRNA), and Dicer-2 distinguishes dsRNA substrates by their termini. Blunt termini promote processive cleavage, while 3' overhanging termini are cleaved distributively. To understand this discrimination, we used cryo-electron microscopy to solve structures of Dicer-2 alone and in complex with blunt dsRNA. Whereas the Platform-PAZ domains have been considered the only Dicer domains that bind dsRNA termini, unexpectedly, we found that the helicase domain is required for binding blunt, but not 3' overhanging, termini. We further showed that blunt dsRNA is locally unwound and threaded through the helicase domain in an adenosine triphosphate-dependent manner. Our studies reveal a previously unrecognized mechanism for optimizing antiviral defense and set the stage for the discovery of helicase-dependent functions in other Dicers.

PubMed: 29269422
DOI: 10.1126/science.aaq0921

実験手法

ELECTRON MICROSCOPY (6.8 Å)