6BSB

Crystal structure of the Mucin-1 SEA domain, L1105M mutant, Selenium-derivative

6BSB の概要

• エントリーDOI: 10.2210/pdb6bsb/pdb
• 分子名称: Mucin-1 (3 entities in total)
• 機能のキーワード: sea domain autoproteolysis muc1, structural protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 12930.99

構造登録者

Noguera, M.E.,Jakoncic, J.,Ermacora, M.R. (登録日: 2017-12-01, 公開日: 2018-12-05, 最終更新日: 2024-10-30)

主引用文献

Noguera, M.E.,Jakoncic, J.,Ermacora, M.R.
High-resolution structure of intramolecularly proteolyzed human mucin-1 SEA domain.
Biochim Biophys Acta Proteins Proteom, 1868:140361-140361, 2020

PubMed Abstract: SEA domains are ubiquitous in large proteins associated with highly glycosylated environments. Certain SEA domains undergo intramolecular proteolysis involving a nucleophilic attack of a serine hydroxyl group on the preceding glycine carbonyl. The mucin-1 (MUC1) SEA domain has been extensively investigated as a model of intramolecular proteolysis. Since neither a general base, a general acid, nor an oxyanion hole could be identified in MUC1 SEA, it has been suggested that proteolysis is accelerated by a non-planarity of the scissile peptide bond imposed by protein folding. A reactant distorted peptide bond has been also invoked to explain the autoproteolysis of several unrelated proteins. However, the only evidence of peptide distortion in MUC1 SEA stems from molecular dynamic simulations of the reactant modeled upon a single NMR structure of the cleaved product. We report the first high-resolution X-ray structure of cleaved MUC1 SEA. Structural comparison with uncleaved SEA domains suggests that the number of residues evolutionarily inserted in the cleaved loop of MUC1 SEA precludes the formation of a properly hydrogen-bonded beta turn. By sequence analysis, we show that this conformational frustration is shared by all known cleaved SEA domains. In addition, alternative conformations of the uncleaved precursor could be modeled in which the scissile peptide bond is planar. The implications of these structures for autoproteolysis are discussed in the light of the previous research on autoproteolysis.

PubMed: 31923589
DOI: 10.1016/j.bbapap.2020.140361

実験手法

X-RAY DIFFRACTION (1.6 Å)