6BS4

Crystal structure of ATPgammaS-bound bacterial Get3-like A and B in Mycobacterium tuberculosis

6BS4 の概要

• エントリーDOI: 10.2210/pdb6bs4/pdb
• 分子名称: Putative ATPase Rv3679, Anion transporter, PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER, ... (6 entities in total)
• 機能のキーワード: complex, unknown function
• 由来する生物種: Mycobacterium tuberculosis H37Rv; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 79410.86

構造登録者

Li, H.,Hu, K.,Kovach, A. (登録日: 2017-12-01, 公開日: 2019-05-15, 最終更新日: 2023-10-04)

主引用文献

Hu, K.,Jordan, A.T.,Zhang, S.,Dhabaria, A.,Kovach, A.,Rangel, M.V.,Ueberheide, B.,Li, H.,Darwin, K.H.
Characterization of Guided Entry of Tail-Anchored Proteins 3 Homologues in Mycobacterium tuberculosis.
J.Bacteriol., 201:-, 2019

PubMed Abstract: We characterized an operon in , Rv3679-Rv3680, in which each open reading frame is annotated to encode "anion transporter ATPase" homologues. Using structure prediction modeling, we found that Rv3679 and Rv3680 more closely resemble the uided ntry of ail-anchored proteins (Get3) chaperone in eukaryotes. Get3 delivers proteins into the membranes of the endoplasmic reticulum and is essential for the normal growth and physiology of some eukaryotes. We sought to characterize the structures of Rv3679 and Rv3680 and test if they have a role in pathogenesis. We solved crystal structures of the nucleotide-bound Rv3679-Rv3680 complex at 2.5 to 3.2 Å and show that while it has some similarities to Get3 and ArsA, there are notable differences, including that these proteins are unlikely to be involved in anion transport. Deletion of both genes did not reveal any conspicuous growth defects or in mice. Collectively, we identified a new class of proteins in bacteria with similarity to Get3 complexes, the functions of which remain to be determined. Numerous bacterial species encode proteins predicted to have similarity with Get3- and ArsA-type anion transporters. Our studies provide evidence that these proteins, which we named BagA and BagB, are unlikely to be involved in anion transport. In addition, BagA and BagB are conserved in all mycobacterial species, including the causative agent of leprosy, which has a highly decayed genome. This conservation suggests that BagAB constitutes a part of the core mycobacterial genome and is needed for some yet-to-be-determined part of the life cycle of these organisms.

PubMed: 31036728
DOI: 10.1128/JB.00159-19

実験手法

X-RAY DIFFRACTION (2.5 Å)