6BRI

RHCC with unreduced and reduced Mercury complexes

6BRI の概要

• エントリーDOI: 10.2210/pdb6bri/pdb
• 分子名称: Right Handed Coiled Coil, MERCURY (II) ION, IODIDE ION, ... (9 entities in total)
• 機能のキーワード: archaea, coiled-coil, nanotube, nanoparticle, mercury, metal binding protein
• 由来する生物種: Staphylothermus marinus F1
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 27663.98

構造登録者

McDougall, M.,Trieu, B.,Stetefeld, J. (登録日: 2017-11-30, 公開日: 2018-02-07, 最終更新日: 2023-10-04)

主引用文献

McDougall, M.,McEleney, K.,Francisco, O.,Trieu, B.,Ogbomo, E.K.,Tomy, G.,Stetefeld, J.
Reductive power of the archaea right-handed coiled coil nanotube (RHCC-NT) and incorporation of mercury clusters inside protein cages.
J. Struct. Biol., 203:281-287, 2018

PubMed Abstract: Coiled coils are well described as powerful oligomerization motifs and exhibit a large diversity of functions, including gene regulation, cell division, membrane fusion and drug extrusion. The archaea S-layer originated right-handed coiled coil -RHCC-NT- is characterized by extreme stability and is free of cysteine and histidine moieties. In the current study, we have followed a multidisciplinary approach to investigate the capacity of RHCC-NT to bind a variety of ionic complex metal ions. At the outside of the RHCC-NT, one mercury ion forms an electrostatic interaction with the S-methyl moiety of the single methionine residue present in each coil. We demonstrate that RHCC-NT is reducing and incorporating metallic mercury in the large-sized interior cavities which are lined up along the tetrameric channel.

PubMed: 29879486
DOI: 10.1016/j.jsb.2018.05.013

実験手法

X-RAY DIFFRACTION (3.266 Å)