6BQN

Cryo-EM structure of ENaC

6BQN の概要

• エントリーDOI: 10.2210/pdb6bqn/pdb
• EMDBエントリー: 7130
• 分子名称: SCNN1A, SCNN1B, EGFP-SCNN1G chimera, ... (7 entities in total)
• 機能のキーワード: sodium channel, membrane protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 203621.73

構造登録者

Noreng, S.,Bharadwaj, A.,Posert, R.,Yoshioka, C.,Baconguis, I. (登録日: 2017-11-28, 公開日: 2018-10-10, 最終更新日: 2025-05-14)

主引用文献

Noreng, S.,Bharadwaj, A.,Posert, R.,Yoshioka, C.,Baconguis, I.
Structure of the human epithelial sodium channel by cryo-electron microscopy.
Elife, 7:-, 2018

PubMed Abstract: The epithelial sodium channel (ENaC), a member of the ENaC/DEG superfamily, regulates Na and water homeostasis. ENaCs assemble as heterotrimeric channels that harbor protease-sensitive domains critical for gating the channel. Here, we present the structure of human ENaC in the uncleaved state determined by single-particle cryo-electron microscopy. The ion channel is composed of a large extracellular domain and a narrow transmembrane domain. The structure reveals that ENaC assembles with a 1:1:1 stoichiometry of α:β:γ subunits arranged in a counter-clockwise manner. The shape of each subunit is reminiscent of a hand with key gating domains of a 'finger' and a 'thumb.' Wedged between these domains is the elusive protease-sensitive inhibitory domain poised to regulate conformational changes of the 'finger' and 'thumb'; thus, the structure provides the first view of the architecture of inhibition of ENaC.

PubMed: 30251954
DOI: 10.7554/eLife.39340

実験手法

ELECTRON MICROSCOPY (3.9 Å)