6BJX
Group I self-splicing intron P4-P6 domain mutant U131A (with isopropanol soaking)
Summary for 6BJX
| Entry DOI | 10.2210/pdb6bjx/pdb |
| Descriptor | Group I self-splicing intron P4-P6 domain, MAGNESIUM ION (2 entities in total) |
| Functional Keywords | ribozyme, rna |
| Biological source | Tetrahymena thermophila |
| Total number of polymer chains | 2 |
| Total formula weight | 102634.83 |
| Authors | Shoffner, G.M. (deposition date: 2017-11-07, release date: 2018-07-04, Last modification date: 2023-10-04) |
| Primary citation | Shoffner, G.M.,Wang, R.,Podell, E.,Cech, T.R.,Guo, F. In Crystallo Selection to Establish New RNA Crystal Contacts. Structure, 26:1275-, 2018 Cited by PubMed Abstract: Crystallography is a major technique for determining large RNA structures. Obtaining diffraction-quality crystals has been the bottleneck. Although several RNA crystallization methods have been developed, the field strongly needs additional approaches. Here we invented an in crystallo selection strategy for identifying mutations that enhance a target RNA's crystallizability. The strategy includes constructing an RNA pool containing random mutations, obtaining crystals, and amplifying the sequences enriched by crystallization. We demonstrated a proof-of-principle application to the P4-P6 domain from the Tetrahymena ribozyme. We further determined the structures of four selected mutants. All four establish new crystal lattice contacts while maintaining the native structure. Three mutants achieve this by relocating bulges and one by making a helix more flexible. In crystallo selection provides opportunities to improve crystals of RNAs or RNA-ligand complexes. Our results also suggest that mutants may be rationally designed for crystallization by "walking" a bulge along the RNA chain. PubMed: 29910185DOI: 10.1016/j.str.2018.05.005 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.14 Å) |
Structure validation
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