6BI4

2.9 Angstrom Resolution Crystal Structure of dTDP-Glucose 4,6-dehydratase (rfbB) from Bacillus anthracis str. Ames in Complex with NAD.

Replaces:  4EGB

Summary for 6BI4

• Entry DOI: 10.2210/pdb6bi4/pdb
• Related PRD ID: PRD_900003
• Descriptor: dTDP-glucose 4,6-dehydratase, beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose, NICKEL (II) ION, ... (6 entities in total)
• Functional Keywords: structural genomics, center for structural genomics of infectious diseases, csgid, dtdp-glucose 4, 6-dehydratase, nad, lyase
• Biological source: Bacillus anthracis str. Ames
• Total number of polymer chains: 4
• Total formula weight: 164516.55

Authors

Halavaty, A.S.,Kuhn, M.,Shuvalova, L.,Minasov, G.,Anderson, W.F.,Center for Structural Genomics of Infectious Diseases (CSGID) (deposition date: 2017-10-31, release date: 2017-11-08, Last modification date: 2024-10-16)

Primary citation

Gokey, T.,Halavaty, A.S.,Minasov, G.,Anderson, W.F.,Kuhn, M.L.
Structure of the Bacillus anthracis dTDP-l-rhamnose biosynthetic pathway enzyme: dTDP-alpha-d-glucose 4,6-dehydratase, RfbB.
J.Struct.Biol., 202:175-181, 2018

PubMed Abstract: Many bacteria require l-rhamnose as a key cell wall component. This sugar is transferred to the cell wall using an activated donor dTDP-l-rhamnose, which is produced by the dTDP-l-rhamnose biosynthetic pathway. We determined the crystal structure of the second enzyme of this pathway dTDP-α-d-glucose 4,6-dehydratase (RfbB) from Bacillus anthracis. Interestingly, RfbB only crystallized in the presence of the third enzyme of the pathway RfbC; however, RfbC was not present in the crystal. Our work represents the first complete structural characterization of the four proteins of this pathway in a single Gram-positive bacterium.

PubMed: 29331609
DOI: 10.1016/j.jsb.2018.01.006

Experimental method

X-RAY DIFFRACTION (2.91 Å)