6BI4

2.9 Angstrom Resolution Crystal Structure of dTDP-Glucose 4,6-dehydratase (rfbB) from Bacillus anthracis str. Ames in Complex with NAD.

「4EGB」から置き換えられました

6BI4 の概要

• エントリーDOI: 10.2210/pdb6bi4/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900003
• 分子名称: dTDP-glucose 4,6-dehydratase, beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose, NICKEL (II) ION, ... (6 entities in total)
• 機能のキーワード: structural genomics, center for structural genomics of infectious diseases, csgid, dtdp-glucose 4, 6-dehydratase, nad, lyase
• 由来する生物種: Bacillus anthracis str. Ames
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 164516.55

構造登録者

Halavaty, A.S.,Kuhn, M.,Shuvalova, L.,Minasov, G.,Anderson, W.F.,Center for Structural Genomics of Infectious Diseases (CSGID) (登録日: 2017-10-31, 公開日: 2017-11-08, 最終更新日: 2024-10-16)

主引用文献

Gokey, T.,Halavaty, A.S.,Minasov, G.,Anderson, W.F.,Kuhn, M.L.
Structure of the Bacillus anthracis dTDP-l-rhamnose biosynthetic pathway enzyme: dTDP-alpha-d-glucose 4,6-dehydratase, RfbB.
J.Struct.Biol., 202:175-181, 2018

PubMed Abstract: Many bacteria require l-rhamnose as a key cell wall component. This sugar is transferred to the cell wall using an activated donor dTDP-l-rhamnose, which is produced by the dTDP-l-rhamnose biosynthetic pathway. We determined the crystal structure of the second enzyme of this pathway dTDP-α-d-glucose 4,6-dehydratase (RfbB) from Bacillus anthracis. Interestingly, RfbB only crystallized in the presence of the third enzyme of the pathway RfbC; however, RfbC was not present in the crystal. Our work represents the first complete structural characterization of the four proteins of this pathway in a single Gram-positive bacterium.

PubMed: 29331609
DOI: 10.1016/j.jsb.2018.01.006

実験手法

X-RAY DIFFRACTION (2.91 Å)