6BCS

LilrB2 D1D2 domains complexed with benzamidine

6BCS の概要

• エントリーDOI: 10.2210/pdb6bcs/pdb
• 分子名称: Leukocyte immunoglobulin-like receptor subfamily B member 2, BENZAMIDINE, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: receptor, ig like domains, immune system
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22773.67

構造登録者

Cao, Q.,Sawaya, M.R.,Eisenberg, D.S. (登録日: 2017-10-20, 公開日: 2018-09-05, 最終更新日: 2024-10-23)

主引用文献

Cao, Q.,Shin, W.S.,Chan, H.,Vuong, C.K.,Dubois, B.,Li, B.,Murray, K.A.,Sawaya, M.R.,Feigon, J.,Black, D.L.,Eisenberg, D.S.,Jiang, L.
Inhibiting amyloid-beta cytotoxicity through its interaction with the cell surface receptor LilrB2 by structure-based design.
Nat Chem, 10:1213-1221, 2018

PubMed Abstract: Inhibiting the interaction between amyloid-β (Aβ) and a neuronal cell surface receptor, LilrB2, has been suggested as a potential route for treating Alzheimer's disease. Supporting this approach, Alzheimer's-like symptoms are reduced in mouse models following genetic depletion of the LilrB2 homologue. In its pathogenic, oligomeric state, Aβ binds to LilrB2, triggering a pathway to synaptic loss. Here we identify the LilrB2 binding moieties of Aβ (KLVFFA) and identify its binding site on LilrB2 from a crystal structure of LilrB2 immunoglobulin domains D1D2 complexed to small molecules that mimic phenylalanine residues. In this structure, we observed two pockets that can accommodate the phenylalanine side chains of KLVFFA. These pockets were confirmed to be KLVFFA binding sites by mutagenesis. Rosetta docking revealed a plausible geometry for the Aβ-LilrB2 complex and assisted with the structure-guided selection of small molecule inhibitors. These molecules inhibit Aβ-LilrB2 interactions in vitro and on the cell surface and reduce Aβ cytotoxicity, which suggests these inhibitors are potential therapeutic leads against Alzheimer's disease.

PubMed: 30297750
DOI: 10.1038/s41557-018-0147-z

実験手法

X-RAY DIFFRACTION (2.1 Å)