6BA6

Solution structure of Rap1b/talin complex

6BA6 の概要

• エントリーDOI: 10.2210/pdb6ba6/pdb
• NMR情報: BMRB: 30353
• 分子名称: Talin-1, Ras-related protein Rap-1b (2 entities in total)
• 機能のキーワード: complex, small gtpase, ubiquitin-like fold, cell adhesion
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29808.03

構造登録者

Zhu, L.,Yang, J.,Qin, J. (登録日: 2017-10-12, 公開日: 2017-12-06, 最終更新日: 2024-05-01)

主引用文献

Zhu, L.,Yang, J.,Bromberger, T.,Holly, A.,Lu, F.,Liu, H.,Sun, K.,Klapproth, S.,Hirbawi, J.,Byzova, T.V.,Plow, E.F.,Moser, M.,Qin, J.
Structure of Rap1b bound to talin reveals a pathway for triggering integrin activation.
Nat Commun, 8:1744-1744, 2017

PubMed Abstract: Activation of transmembrane receptor integrin by talin is essential for inducing cell adhesion. However, the pathway that recruits talin to the membrane, which critically controls talin's action, remains elusive. Membrane-anchored mammalian small GTPase Rap1 is known to bind talin-F0 domain but the binding was shown to be weak and thus hardly studied. Here we show structurally that talin-F0 binds to human Rap1b like canonical Rap1 effectors despite little sequence homology, and disruption of the binding strongly impairs integrin activation, cell adhesion, and cell spreading. Furthermore, while being weak in conventional binary binding conditions, the Rap1b/talin interaction becomes strong upon attachment of activated Rap1b to vesicular membranes that mimic the agonist-induced microenvironment. These data identify a crucial Rap1-mediated membrane-targeting mechanism for talin to activate integrin. They further broadly caution the analyses of weak protein-protein interactions that may be pivotal for function but neglected in the absence of specific cellular microenvironments.

PubMed: 29170462
DOI: 10.1038/s41467-017-01822-8

実験手法

SOLUTION NMR