6B9S

MPnS crystallized in the absence of substrate

6B9S の概要

• エントリーDOI: 10.2210/pdb6b9s/pdb
• 分子名称: Methylphosphonate synthase, FE (III) ION (3 entities in total)
• 機能のキーワード: phosphonate, methylphosphonate, iron, oxidoreductase
• 由来する生物種: Nitrosopumilus maritimus SCM1
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 417193.94

構造登録者

Born, D.A.,Drennan, C.L. (登録日: 2017-10-11, 公開日: 2017-12-20, 最終更新日: 2024-03-13)

主引用文献

Born, D.A.,Ulrich, E.C.,Ju, K.S.,Peck, S.C.,van der Donk, W.A.,Drennan, C.L.
Structural basis for methylphosphonate biosynthesis.
Science, 358:1336-1339, 2017

PubMed Abstract: Methylphosphonate synthase (MPnS) produces methylphosphonate, a metabolic precursor to methane in the upper ocean. Here, we determine a 2.35-angstrom resolution structure of MPnS and discover that it has an unusual 2-histidine-1-glutamine iron-coordinating triad. We further solve the structure of a related enzyme, hydroxyethylphosphonate dioxygenase from (HEPD), and find that it displays the same motif. HEPD can be converted into an MPnS by mutation of glutamine-adjacent residues, identifying the molecular requirements for methylphosphonate synthesis. Using these sequence markers, we find numerous putative MPnSs in marine microbiomes and confirm that MPnS is present in the abundant The ubiquity of MPnS-containing microbes supports the proposal that methylphosphonate is a source of methane in the upper, aerobic ocean, where phosphorus-starved microbes catabolize methylphosphonate for its phosphorus.

PubMed: 29217579
DOI: 10.1126/science.aao3435

実験手法

X-RAY DIFFRACTION (2.373 Å)