6B8N

Crystal Structure of the Ca2+/CaM:Kv7.4 (KCNQ4) AB Domain Complex, 10 uM CaCl2 soak

6B8N の概要

• エントリーDOI: 10.2210/pdb6b8n/pdb
• 分子名称: Potassium voltage-gated channel subfamily KQT member 4, Calmodulin-1, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: ion channel, complex, metal transport
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 107609.68

構造登録者

Chang, A.,Minor, D.L. (登録日: 2017-10-09, 公開日: 2018-03-14, 最終更新日: 2023-10-04)

主引用文献

Chang, A.,Abderemane-Ali, F.,Hura, G.L.,Rossen, N.D.,Gate, R.E.,Minor, D.L.
A Calmodulin C-Lobe Ca
Neuron, 97:836-852.e6, 2018

PubMed Abstract: Kv7 (KCNQ) voltage-gated potassium channels control excitability in the brain, heart, and ear. Calmodulin (CaM) is crucial for Kv7 function, but how this calcium sensor affects activity has remained unclear. Here, we present X-ray crystallographic analysis of CaM:Kv7.4 and CaM:Kv7.5 AB domain complexes that reveal an Apo/CaM clamp conformation and calcium binding preferences. These structures, combined with small-angle X-ray scattering, biochemical, and functional studies, establish a regulatory mechanism for Kv7 CaM modulation based on a common architecture in which a CaM C-lobe calcium-dependent switch releases a shared Apo/CaM clamp conformation. This C-lobe switch inhibits voltage-dependent activation of Kv7.4 and Kv7.5 but facilitates Kv7.1, demonstrating that mechanism is shared by Kv7 isoforms despite the different directions of CaM modulation. Our findings provide a unified framework for understanding how CaM controls different Kv7 isoforms and highlight the role of membrane proximal domains for controlling voltage-gated channel function. VIDEO ABSTRACT.

PubMed: 29429937
DOI: 10.1016/j.neuron.2018.01.035

実験手法

X-RAY DIFFRACTION (2.201 Å)