6B2V

Pyran synthase domain from module nine of the sorangicin pathway

6B2V の概要

• エントリーDOI: 10.2210/pdb6b2v/pdb
• 分子名称: SorB (2 entities in total)
• 機能のキーワード: polyketide, pyran, trans-at, antibiotic, transferase
• 由来する生物種: Sorangium cellulosum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32801.00

構造登録者

Wagner, D.T.,Keatinge-Clay, A.T. (登録日: 2017-09-20, 公開日: 2018-04-18, 最終更新日: 2024-03-13)

主引用文献

Wagner, D.T.,Zhang, Z.,Meoded, R.A.,Cepeda, A.J.,Piel, J.,Keatinge-Clay, A.T.
Structural and Functional Studies of a Pyran Synthase Domain from a trans-Acyltransferase Assembly Line.
ACS Chem. Biol., 13:975-983, 2018

PubMed Abstract: trans-Acyltransferase assembly lines possess enzymatic domains often not observed in their better characterized cis-acyltransferase counterparts. Within this repertoire of largely unexplored biosynthetic machinery is a class of enzymes called the pyran synthases that catalyze the formation of five- and six-membered cyclic ethers from diverse polyketide chains. The 1.55 Å resolution crystal structure of a pyran synthase domain excised from the ninth module of the sorangicin assembly line highlights the similarity of this enzyme to the ubiquitous dehydratase domain and provides insight into the mechanism of ring formation. Functional assays of point mutants reveal the central importance of the active site histidine that is shared with the dehydratases as well as the supporting role of a neighboring semiconserved asparagine.

PubMed: 29481043
DOI: 10.1021/acschembio.8b00049

実験手法

X-RAY DIFFRACTION (1.55 Å)