6B25

Crystal structure of human STAC1 Tandem SH3 Domains (288-402)

6B25 の概要

• エントリーDOI: 10.2210/pdb6b25/pdb
• 分子名称: SH3 and cysteine-rich domain-containing protein, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID (3 entities in total)
• 機能のキーワード: excitation-contraction coupling, ion channel adaptor protein, protein binding
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13920.81

構造登録者

Wong King Yuen, S.M.,Van Petegem, F. (登録日: 2017-09-19, 公開日: 2017-10-25, 最終更新日: 2024-03-13)

主引用文献

Wong King Yuen, S.M.,Campiglio, M.,Tung, C.C.,Flucher, B.E.,Van Petegem, F.
Structural insights into binding of STAC proteins to voltage-gated calcium channels.
Proc. Natl. Acad. Sci. U.S.A., 114:E9520-E9528, 2017

PubMed Abstract: Excitation-contraction (EC) coupling in skeletal muscle requires functional and mechanical coupling between L-type voltage-gated calcium channels (Ca1.1) and the ryanodine receptor (RyR1). Recently, STAC3 was identified as an essential protein for EC coupling and is part of a group of three proteins that can bind and modulate L-type voltage-gated calcium channels. Here, we report crystal structures of tandem-SH3 domains of different STAC isoforms up to 1.2-Å resolution. These form a rigid interaction through a conserved interdomain interface. We identify the linker connecting transmembrane repeats II and III in two different Ca isoforms as a binding site for the SH3 domains and report a crystal structure of the complex with the STAC2 isoform. The interaction site includes the location for a disease variant in STAC3 that has been linked to Native American myopathy (NAM). Introducing the mutation does not cause misfolding of the SH3 domains, but abolishes the interaction. Disruption of the interaction via mutations in the II-III loop perturbs skeletal muscle EC coupling, but preserves the ability of STAC3 to slow down inactivation of Ca1.2.

PubMed: 29078335
DOI: 10.1073/pnas.1708852114

実験手法

X-RAY DIFFRACTION (2.39 Å)