6AX2
Solution structure of Magi3 a specific insect toxin from the spider Macrothele gigas
Summary for 6AX2
| Entry DOI | 10.2210/pdb6ax2/pdb |
| NMR Information | BMRB: 30339 |
| Descriptor | Mu-hexatoxin-Mg2a (1 entity in total) |
| Functional Keywords | refolding, spider toxin, insecticidal peptide, toxin |
| Biological source | Macrothele gigas (Spider) |
| Cellular location | Secreted : P83559 |
| Total number of polymer chains | 1 |
| Total formula weight | 5241.11 |
| Authors | del Rio-Portilla, F.,Titaux-Delgado, G.A.,Escobedo-Gonzalez, F.C. (deposition date: 2017-09-06, release date: 2018-01-10, Last modification date: 2024-10-23) |
| Primary citation | Titaux-Delgado, G.,Carrillo, E.,Mendoza, A.,Mayorga-Flores, M.,Escobedo-Gonzalez, F.C.,Cano-Sanchez, P.,Lopez-Vera, E.,Corzo, G.,Del Rio-Portilla, F. Successful refolding and NMR structure of rMagi3: A disulfide-rich insecticidal spider toxin. Protein Sci., 27:692-701, 2018 Cited by PubMed Abstract: The need for molecules with high specificity against noxious insects leads the search towards spider venoms that have evolved highly selective toxins for insect preys. In this respect, spiders as a highly diversified group of almost exclusive insect predators appear to possess infinite potential for the discovery of novel insect-selective toxins. In 2003, a group of toxins was isolated from the spider Macrothele gigas and the amino acid sequence was reported. We obtained, by molecular biology techniques in a heterologous system, one of these toxins. Purification process was optimized by chromatographic methods to determine the three-dimensional structure by nuclear magnetic resonance in solution, and, finally, their biological activity was tested. rMagi3 resulted to be a specific insect toxin with no effect on mice. PubMed: 29247580DOI: 10.1002/pro.3363 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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