6AX2

Solution structure of Magi3 a specific insect toxin from the spider Macrothele gigas

6AX2 の概要

• エントリーDOI: 10.2210/pdb6ax2/pdb
• NMR情報: BMRB: 30339
• 分子名称: Mu-hexatoxin-Mg2a (1 entity in total)
• 機能のキーワード: refolding, spider toxin, insecticidal peptide, toxin
• 由来する生物種: Macrothele gigas (Spider)
• 細胞内の位置: Secreted : P83559
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5241.11

構造登録者

del Rio-Portilla, F.,Titaux-Delgado, G.A.,Escobedo-Gonzalez, F.C. (登録日: 2017-09-06, 公開日: 2018-01-10, 最終更新日: 2024-10-23)

主引用文献

Titaux-Delgado, G.,Carrillo, E.,Mendoza, A.,Mayorga-Flores, M.,Escobedo-Gonzalez, F.C.,Cano-Sanchez, P.,Lopez-Vera, E.,Corzo, G.,Del Rio-Portilla, F.
Successful refolding and NMR structure of rMagi3: A disulfide-rich insecticidal spider toxin.
Protein Sci., 27:692-701, 2018

PubMed Abstract: The need for molecules with high specificity against noxious insects leads the search towards spider venoms that have evolved highly selective toxins for insect preys. In this respect, spiders as a highly diversified group of almost exclusive insect predators appear to possess infinite potential for the discovery of novel insect-selective toxins. In 2003, a group of toxins was isolated from the spider Macrothele gigas and the amino acid sequence was reported. We obtained, by molecular biology techniques in a heterologous system, one of these toxins. Purification process was optimized by chromatographic methods to determine the three-dimensional structure by nuclear magnetic resonance in solution, and, finally, their biological activity was tested. rMagi3 resulted to be a specific insect toxin with no effect on mice.

PubMed: 29247580
DOI: 10.1002/pro.3363

実験手法

SOLUTION NMR