6AW2

Crystal structure of the HopQ-CEACAM1 complex

Summary for 6AW2

• Entry DOI: 10.2210/pdb6aw2/pdb
• Descriptor: Carcinoembryonic antigen-related cell adhesion molecule 1, HopQ (3 entities in total)
• Functional Keywords: cell adhesion
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 2
• Total formula weight: 59129.05

Authors

Bonsor, D.A.,Sundberg, E.J. (deposition date: 2017-09-05, release date: 2018-05-16, Last modification date: 2023-10-04)

Primary citation

Bonsor, D.A.,Zhao, Q.,Schmidinger, B.,Weiss, E.,Wang, J.,Deredge, D.,Beadenkopf, R.,Dow, B.,Fischer, W.,Beckett, D.,Wintrode, P.L.,Haas, R.,Sundberg, E.J.
TheHelicobacter pyloriadhesin protein HopQ exploits the dimer interface of human CEACAMs to facilitate translocation of the oncoprotein CagA.
EMBO J., 37:-, 2018

PubMed Abstract: infects half of the world's population, and strains that encode the type IV secretion system for injection of the oncoprotein CagA into host gastric epithelial cells are associated with elevated levels of cancer. CagA translocation into host cells is dependent on interactions between the adhesin protein HopQ and human CEACAMs. Here, we present high-resolution structures of several HopQ-CEACAM complexes and CEACAMs in their monomeric and dimeric forms establishing that HopQ uses a coupled folding and binding mechanism to engage the canonical CEACAM dimerization interface for CEACAM recognition. By combining mutagenesis with biophysical and functional analyses, we show that the modes of CEACAM recognition by HopQ and CEACAMs themselves are starkly different. Our data describe precise molecular mechanisms by which microbes exploit host CEACAMs for infection and enable future development of novel oncoprotein translocation inhibitors and -specific antimicrobial agents.

PubMed: 29724755
DOI: 10.15252/embj.201798664

Experimental method

X-RAY DIFFRACTION (2.68 Å)