6AVB

CryoEM structure of Mical Oxidized Actin (Class 1)

6AVB の概要

• エントリーDOI: 10.2210/pdb6avb/pdb
• 関連するPDBエントリー: 5AV9
• EMDBエントリー: 7007 7008
• 分子名称: Actin, alpha skeletal muscle, ADENOSINE-5'-DIPHOSPHATE (2 entities in total)
• 機能のキーワード: f-actin, mical, cytoskeleton, helix, structural protein
• 由来する生物種: Oryctolagus cuniculus (Rabbit)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 127707.52

構造登録者

Grintsevich, E.E.,Ge, P.,Sawaya, M.R.,Yesilyurt, H.G.,Terman, J.R.,Zhou, Z.H.,Reisler, E. (登録日: 2017-09-01, 公開日: 2018-01-17, 最終更新日: 2025-05-21)

主引用文献

Grintsevich, E.E.,Ge, P.,Sawaya, M.R.,Yesilyurt, H.G.,Terman, J.R.,Zhou, Z.H.,Reisler, E.
Catastrophic disassembly of actin filaments via Mical-mediated oxidation.
Nat Commun, 8:2183-2183, 2017

PubMed Abstract: Actin filament assembly and disassembly are vital for cell functions. MICAL Redox enzymes are important post-translational effectors of actin that stereo-specifically oxidize actin's M44 and M47 residues to induce cellular F-actin disassembly. Here we show that Mical-oxidized (Mox) actin can undergo extremely fast (84 subunits/s) disassembly, which depends on F-actin's nucleotide-bound state. Using near-atomic resolution cryoEM reconstruction and single filament TIRF microscopy we identify two dynamic and structural states of Mox-actin. Modeling actin's D-loop region based on our 3.9 Å cryoEM reconstruction suggests that oxidation by Mical reorients the side chain of M44 and induces a new intermolecular interaction of actin residue M47 (M47-O-T351). Site-directed mutagenesis reveals that this interaction promotes Mox-actin instability. Moreover, we find that Mical oxidation of actin allows for cofilin-mediated severing even in the presence of inorganic phosphate. Thus, in conjunction with cofilin, Mical oxidation of actin promotes F-actin disassembly independent of the nucleotide-bound state.

PubMed: 29259197
DOI: 10.1038/s41467-017-02357-8

実験手法

ELECTRON MICROSCOPY (3.9 Å)