6AP7

Crystal Structure of DAD2 in complex with 2-(2-methyl-3-nitroanilino)benzoic acid

6AP7 の概要

• エントリーDOI: 10.2210/pdb6ap7/pdb
• 関連するPDBエントリー: 4DNP
• 分子名称: Probable strigolactone esterase DAD2, 2-[(2-methyl-3-nitrophenyl)amino]benzoic acid, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: alpha/beta hydrolase, plant protein
• 由来する生物種: Petunia hybrida (Petunia)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 60408.93

構造登録者

Hamiaux, C. (登録日: 2017-08-17, 公開日: 2018-03-21, 最終更新日: 2023-10-04)

主引用文献

Hamiaux, C.,Drummond, R.S.M.,Luo, Z.,Lee, H.W.,Sharma, P.,Janssen, B.J.,Perry, N.B.,Denny, W.A.,Snowden, K.C.
Inhibition of strigolactone receptors byN-phenylanthranilic acid derivatives: Structural and functional insights.
J. Biol. Chem., 293:6530-6543, 2018

PubMed Abstract: The strigolactone (SL) family of plant hormones regulates a broad range of physiological processes affecting plant growth and development and also plays essential roles in controlling interactions with parasitic weeds and symbiotic fungi. Recent progress elucidating details of SL biosynthesis, signaling, and transport offers many opportunities for discovering new plant-growth regulators via chemical interference. Here, using high-throughput screening and downstream biochemical assays, we identified -phenylanthranilic acid derivatives as potent inhibitors of the SL receptors from petunia (DAD2), rice (OsD14), and (AtD14). Crystal structures of DAD2 and OsD14 in complex with inhibitors further provided detailed insights into the inhibition mechanism, and modeling of 19 other plant strigolactone receptors suggested that these compounds are active across a large range of plant species. Altogether, these results provide chemical tools for investigating SL signaling and further define a framework for structure-based approaches to design and validate optimized inhibitors of SL receptors for specific plant targets.

PubMed: 29523686
DOI: 10.1074/jbc.RA117.001154

実験手法

X-RAY DIFFRACTION (1.51 Å)