6ALY

Solution structure of yeast Med15 ABD2 residues 277-368

6ALY の概要

• エントリーDOI: 10.2210/pdb6aly/pdb
• NMR情報: BMRB: 30330
• 分子名称: Mediator of RNA polymerase II transcription subunit 15 (1 entity in total)
• 機能のキーワード: mediator, transcription activation, helical, abd, med15, gal11, yeast, transcription
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10525.71

構造登録者

Tuttle, L.M.,Pacheco, D.,Warfield, L.,Hahn, S.,Klevit, R.E. (登録日: 2017-08-08, 公開日: 2018-03-21, 最終更新日: 2024-05-15)

主引用文献

Tuttle, L.M.,Pacheco, D.,Warfield, L.,Luo, J.,Ranish, J.,Hahn, S.,Klevit, R.E.
Gcn4-Mediator Specificity Is Mediated by a Large and Dynamic Fuzzy Protein-Protein Complex.
Cell Rep, 22:3251-3264, 2018

PubMed Abstract: Transcription activation domains (ADs) are inherently disordered proteins that often target multiple coactivator complexes, but the specificity of these interactions is not understood. Efficient transcription activation by yeast Gcn4 requires its tandem ADs and four activator-binding domains (ABDs) on its target, the Mediator subunit Med15. Multiple ABDs are a common feature of coactivator complexes. We find that the large Gcn4-Med15 complex is heterogeneous and contains nearly all possible AD-ABD interactions. Gcn4-Med15 forms via a dynamic fuzzy protein-protein interface, where ADs bind the ABDs in multiple orientations via hydrophobic regions that gain helicity. This combinatorial mechanism allows individual low-affinity and specificity interactions to generate a biologically functional, specific, and higher affinity complex despite lacking a defined protein-protein interface. This binding strategy is likely representative of many activators that target multiple coactivators, as it allows great flexibility in combinations of activators that can cooperate to regulate genes with variable coactivator requirements.

PubMed: 29562181
DOI: 10.1016/j.celrep.2018.02.097

実験手法

SOLUTION NMR