6AKD

Crystal structure of IdnL7

6AKD の概要

• エントリーDOI: 10.2210/pdb6akd/pdb
• 分子名称: AMP-dependent synthetase and ligase, '5'-O-(N-(L-ALANYL)-SULFAMOYL)ADENOSINE, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: ligase, atp-binding, five layered alpha-beta-alpha-beta-alpha sandwich fold
• 由来する生物種: Streptomyces sp. ML694-90F3
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 58116.34

構造登録者

Cieslak, J.,Miyanaga, A.,Kudo, F.,Eguchi, T. (登録日: 2018-08-31, 公開日: 2019-03-06, 最終更新日: 2023-11-22)

主引用文献

Cieslak, J.,Miyanaga, A.,Takaishi, M.,Kudo, F.,Eguchi, T.
Functional and structural characterization of IdnL7, an adenylation enzyme involved in incednine biosynthesis.
Acta Crystallogr F Struct Biol Commun, 75:299-306, 2019

PubMed Abstract: Adenylation enzymes play an important role in the selective incorporation of the cognate carboxylate substrates in natural product biosynthesis. Here, the biochemical and structural characterization of the adenylation enzyme IdnL7, which is involved in the biosynthesis of the macrolactam polyketide antibiotic incednine, is reported. Biochemical analysis showed that IdnL7 selects and activates several small amino acids. The structure of IdnL7 in complex with an L-alanyl-adenylate intermediate mimic, 5'-O-[N-(L-alanyl)sulfamoyl]adenosine, was determined at 2.1 Å resolution. The structure of IdnL7 explains the broad substrate specificity of IdnL7 towards small L-amino acids.

PubMed: 30950831
DOI: 10.1107/S2053230X19002863

実験手法

X-RAY DIFFRACTION (2.1 Å)