6AK0
Solution NMR structure of a new lasso peptide specialicin
Summary for 6AK0
| Entry DOI | 10.2210/pdb6ak0/pdb |
| Descriptor | CYS-LEU-GLY-VAL-GLY-SER-CYS-VAL-ASP-PHE-ALA-GLY-CYS-GLY-TYR-ALA-VAL-VAL-CYS-PHE-DTR (1 entity in total) |
| Functional Keywords | lasso peptide, streptomyces specialis, specialicin, unknown function |
| Biological source | Streptomyces specialis |
| Total number of polymer chains | 1 |
| Total formula weight | 2156.53 |
| Authors | Hemmi, H.,Kodani, S.,Kaweewan, I.,Komaki, H. (deposition date: 2018-08-28, release date: 2018-12-05, Last modification date: 2024-11-06) |
| Primary citation | Kaweewan, I.,Hemmi, H.,Komaki, H.,Harada, S.,Kodani, S. Isolation and structure determination of a new lasso peptide specialicin based on genome mining Bioorg. Med. Chem., 26:6050-6055, 2018 Cited by PubMed Abstract: Based on genome mining, a new lasso peptide specialicin was isolated from the extract of Streptomyces specialis. The structure of specialicin was established by ESI-MS and NMR analyses to be a lasso peptide with the length of 21 amino acids, containing an isopeptide bond and two disulfide bonds in the molecule. The stereochemistries of the constituent amino acids except for Trp were determined to be L and the stereochemistry of Trp at C-terminus was determined to be D. Three dimensional structure of specialicin was determined based on NOE experimental data, which indicated that specialicin possessed the similar conformational structure with siamycin I. Specialicin showed the antibacterial activity against Micrococcus luteus and the moderate anti-HIV activity against HIV-1 NL4-3. The biosynthetic gene cluster of specialicin was proposed from the genome sequence data of S. specialis. PubMed: 30448257DOI: 10.1016/j.bmc.2018.11.007 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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