6AK0

Solution NMR structure of a new lasso peptide specialicin

6AK0 の概要

• エントリーDOI: 10.2210/pdb6ak0/pdb
• 分子名称: CYS-LEU-GLY-VAL-GLY-SER-CYS-VAL-ASP-PHE-ALA-GLY-CYS-GLY-TYR-ALA-VAL-VAL-CYS-PHE-DTR (1 entity in total)
• 機能のキーワード: lasso peptide, streptomyces specialis, specialicin, unknown function
• 由来する生物種: Streptomyces specialis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2156.53

構造登録者

Hemmi, H.,Kodani, S.,Kaweewan, I.,Komaki, H. (登録日: 2018-08-28, 公開日: 2018-12-05, 最終更新日: 2024-11-06)

主引用文献

Kaweewan, I.,Hemmi, H.,Komaki, H.,Harada, S.,Kodani, S.
Isolation and structure determination of a new lasso peptide specialicin based on genome mining
Bioorg. Med. Chem., 26:6050-6055, 2018

PubMed Abstract: Based on genome mining, a new lasso peptide specialicin was isolated from the extract of Streptomyces specialis. The structure of specialicin was established by ESI-MS and NMR analyses to be a lasso peptide with the length of 21 amino acids, containing an isopeptide bond and two disulfide bonds in the molecule. The stereochemistries of the constituent amino acids except for Trp were determined to be L and the stereochemistry of Trp at C-terminus was determined to be D. Three dimensional structure of specialicin was determined based on NOE experimental data, which indicated that specialicin possessed the similar conformational structure with siamycin I. Specialicin showed the antibacterial activity against Micrococcus luteus and the moderate anti-HIV activity against HIV-1 NL4-3. The biosynthetic gene cluster of specialicin was proposed from the genome sequence data of S. specialis.

PubMed: 30448257
DOI: 10.1016/j.bmc.2018.11.007

実験手法

SOLUTION NMR