6AHY

Wnt signaling complex

6AHY の概要

• エントリーDOI: 10.2210/pdb6ahy/pdb
• 分子名称: Frizzled-8, Proto-oncogene Wnt-3, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: wnt signaling, canonical wnt pathway, signaling protein
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 160199.40

構造登録者

Hirai, H.,Arimori, T.,Matoba, K.,Mihara, E.,Takagi, J. (登録日: 2018-08-21, 公開日: 2019-05-01, 最終更新日: 2023-11-22)

主引用文献

Hirai, H.,Matoba, K.,Mihara, E.,Arimori, T.,Takagi, J.
Crystal structure of a mammalian Wnt-frizzled complex.
Nat.Struct.Mol.Biol., 26:372-379, 2019

PubMed Abstract: Wnt signaling plays fundamental roles in organogenesis, tissue regeneration and cancer, but high-resolution structural information of mammalian Wnt proteins is lacking. We solved a 2.8-Å resolution crystal structure of human Wnt3 in complex with mouse Frizzled 8 Cys-rich domain (CRD). Wnt3 grabs the receptor in a manner very similar to that found in Xenopus Wnt8 complexed with the same receptor. Unlike Xenopus Wnt8-bound CRD, however, Wnt3-bound CRD formed a symmetrical dimer in the crystal by exchanging the tip of the unsaturated acyl chain attached to each Wnt3, confirming the ability of Wnt and Frizzled CRD to form a 2:2 complex. The hypervariable 'linker' region of Wnt3 formed a β-hairpin protrusion opposite from the Frizzled binding interface, consistent with its proposed role in the coreceptor recognition. Direct binding between this segment and the Wnt coreceptor LRP6 was confirmed, enabling us to build a structural model of the Wnt-Frizzled-LRP6 ternary complex.

PubMed: 31036956
DOI: 10.1038/s41594-019-0216-z

実験手法

X-RAY DIFFRACTION (2.8 Å)