6AGY

Aspergillus fumigatus Af293 NDK

6AGY の概要

• エントリーDOI: 10.2210/pdb6agy/pdb
• 分子名称: Nucleoside diphosphate kinase (2 entities in total)
• 機能のキーワード: kinase, transferase
• 由来する生物種: Aspergillus fumigatus Af293 (Aspergillus fumigatus)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18023.62

構造登録者

Hu, Y.,Han, L. (登録日: 2018-08-15, 公開日: 2019-03-13, 最終更新日: 2023-11-22)

主引用文献

Hu, Y.,Jia, X.,Lu, Z.,Han, L.
Characterization of crystal structure and key residues of Aspergillus fumigatus nucleoside diphosphate kinase.
Biochem. Biophys. Res. Commun., 511:148-153, 2019

PubMed Abstract: Aspergillus fumigatus is a major pathogen of invasive pulmonary aspergillosis with high mortality rate. The nucleoside diphosphate kinase of A. fumigatus, AfNDK (also called SwoH) is essential for its viability, however, its structural characteristic was unknown. In this study, we solved the crystal structure of AfNDK and found that it exists predominantly in form of tetramer in solution. Oligomeric form rather than dimeric form was essential for its kinase activity. The Arg30 and the C terminal amino acids were crucial for dimer-dimer interaction and the viability of A. fumigatus. Mutation V83F might make the secondary structure α5 helix protrude outward so that the whole protein structure became unstable at higher temperature, which might subsequently result to the inviability of A. fumigatus under 44 °C. In conclusion, the crystal structure of AfNDK was for the first time analyzed and the stability of the tetrameric form with dimer-dimer interaction were crucial for its function in A. fumigatus.

PubMed: 30773256
DOI: 10.1016/j.bbrc.2019.01.126

実験手法

X-RAY DIFFRACTION (1.8 Å)