Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

6AGP

Structure of Rac1 in the low-affinity state for Mg2+

Summary for 6AGP
Entry DOI10.2210/pdb6agp/pdb
DescriptorRas-related C3 botulinum toxin substrate 1, GUANOSINE-5'-DIPHOSPHATE, MAGNESIUM ION (3 entities in total)
Functional Keywordsnucleotide binding, gtpase activity, gtp binding, signaling protein
Biological sourceHomo sapiens (Human)
Total number of polymer chains1
Total formula weight20499.84
Authors
Toyama, Y.,Kontani, K.,Katada, T.,Shimada, I. (deposition date: 2018-08-13, release date: 2019-03-20, Last modification date: 2024-05-01)
Primary citationToyama, Y.,Kontani, K.,Katada, T.,Shimada, I.
Conformational landscape alternations promote oncogenic activities of Ras-related C3 botulinum toxin substrate 1 as revealed by NMR.
Sci Adv, 5:eaav8945-eaav8945, 2019
Cited by
PubMed Abstract: Ras-related C3 botulinum toxin substrate 1 (Rac1) plays critical roles in the maintenance of cell morphology by cycling between inactive guanosine diphosphate (GDP)-bound and active guanosine triphosphate (GTP)-bound states. Rac1 P29S mutant is known to strongly promote oncogenesis by facilitating its intrinsic GDP dissociation and thereby increasing the level of the GTP-bound state. Here, we used solution nuclear magnetic resonance spectroscopy to investigate the activation mechanism of the oncogenic P29S mutant. We demonstrate that the conformational landscape is markedly altered in the mutant, and the preexisting equilibrium is shifted toward the conformation with reduced affinity for Mg a cofactor that is critical for maintaining stable GDP binding. Our results suggest that the alternation of the preexisting conformational equilibrium of proteins is one of the fundamental mechanisms underlying their oncogenic activities.
PubMed: 30891502
DOI: 10.1126/sciadv.aav8945
PDB entries with the same primary citation
Experimental method
SOLUTION NMR
Structure validation

238268

数据于2025-07-02公开中

PDB statisticsPDBj update infoContact PDBjnumon