6ABV
Crystal structure of citrate synthase (Msed_0281) from Metallosphaera sedula
Summary for 6ABV
| Entry DOI | 10.2210/pdb6abv/pdb |
| Descriptor | Citrate synthase, GLYCEROL (3 entities in total) |
| Functional Keywords | citrate synthase, metallosphaera sedula, transferase |
| Biological source | Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2) |
| Total number of polymer chains | 1 |
| Total formula weight | 43813.92 |
| Authors | Lee, S.-H.,Kim, K.-J. (deposition date: 2018-07-24, release date: 2019-01-16, Last modification date: 2023-11-22) |
| Primary citation | Lee, S.H.,Kim, K.J. Crystal structure and biochemical properties of msed_0281, the citrate synthase from Metallosphaera sedula. Biochem. Biophys. Res. Commun., 509:722-727, 2019 Cited by PubMed Abstract: Metallosphaera sedula is a thermoacidophilic archaeon that has carbon fixation ability using the 3-hydroxypropionate/4-hydroxybutyrate(3-HP/4-HB) cycle, and has an incomplete TCA cycle to produce necessary biosynthetic precursors. The citrate synthase from M. sedula (MsCS) is an enzyme involved in the first step of the incomplete TCA cycle, catalyzing the conversion of oxaloacetate and acetyl-CoA into citrate and coenzyme A. To investigate the molecular mechanism of MsCS, we determined its crystal structure at 1.8 Å resolution. As other known CSs, MsCS functions as a dimer, and each monomer consists of two domains, a large domain and a small domain. We also determined the structure of the complex with acetyl-CoA and revealed the acetyl-CoA binding mode of MsCS. Structural comparison of MsCS with another CS in complex with oxaloacetate enabled us to predict the oxaloacetate binding site. Moreover, we performed inhibitory kinetic analyses of MsCS, and showed that the protein is inhibited by citrate and ATP by competitive and non-competitive inhibition modes, respectively, but not by NADH. Based on these results, we suggest that MsCS belongs to the type-I CS with structural and biochemical properties similar to those of CSs involved in the conventional TCA cycle. PubMed: 30611567DOI: 10.1016/j.bbrc.2018.12.172 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
Download full validation report
