6A8V

PhoQ sensor domain (D179R mutant): analysis of internal cavity

6A8V の概要

• エントリーDOI: 10.2210/pdb6a8v/pdb
• 分子名称: Sensor protein PhoQ (2 entities in total)
• 機能のキーワード: sensor, signaling protein
• 由来する生物種: Escherichia coli (strain K12)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34452.84

構造登録者

Yoshitani, K.,Ishii, E.,Taniguchi, K.,Sugimoto, H.,Shiro, Y.,Mori, H.,Akiyama, Y.,Kato, A.,Utsumi, R.,Eguchi, Y. (登録日: 2018-07-10, 公開日: 2019-01-30, 最終更新日: 2023-11-22)

主引用文献

Yoshitani, K.,Ishii, E.,Taniguchi, K.,Sugimoto, H.,Shiro, Y.,Akiyama, Y.,Kato, A.,Utsumi, R.,Eguchi, Y.
Identification of an internal cavity in the PhoQ sensor domain for PhoQ activity and SafA-mediated control.
Biosci. Biotechnol. Biochem., 83:684-694, 2019

PubMed Abstract: The PhoQ/PhoP two-component signal transduction system is conserved in various Gram-negative bacteria and is often involved in the expression of virulence in pathogens. The small inner membrane protein SafA activates PhoQ in Escherichia coli independently from other known signals that control PhoQ activity. We have previously shown that SafA directly interacts with the sensor domain of the periplasmic region of PhoQ (PhoQ-SD) for activation, and that a D179R mutation in PhoQ-SD attenuates PhoQ activation by SafA. In this study, structural comparison of wild-type PhoQ-SD and D179R revealed a difference in the cavity (SD (sensory domain) pocket) found in the central core of this domain. This was the only structural difference between the two proteins. Site-directed mutagenesis of the residues surrounding the SD pocket has supported the SD pocket as a site involved in PhoQ activity. Furthermore, the SD pocket has also been shown to be involved in SafA-mediated PhoQ control.

PubMed: 30632929
DOI: 10.1080/09168451.2018.1562879

実験手法

X-RAY DIFFRACTION (2.7 Å)