6A8L

Crystal structure of nicotinamidase/ pyrazinamidase PncA from Bacillus subtilis

6A8L の概要

• エントリーDOI: 10.2210/pdb6a8l/pdb
• 分子名称: Isochorismatase, ZINC ION (3 entities in total)
• 機能のキーワード: bacillus subtilis, nicotinamidase/pyrazinamidase, hydrolase, metal binding protein
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 41110.60

構造登録者

Shang, F.,Chen, J.,Wang, L.,Xu, Y. (登録日: 2018-07-09, 公開日: 2018-08-01, 最終更新日: 2023-11-22)

主引用文献

Shang, F.,Chen, J.,Wang, L.,Jin, L.,Zou, L.,Bu, T.,Dong, Y.,Ha, N.C.,Nam, K.H.,Quan, C.,Xu, Y.
Crystal structure of the nicotinamidase/pyrazinamidase PncA from Bacillus subtilis.
Biochem. Biophys. Res. Commun., 503:2906-2911, 2018

PubMed Abstract: The nicotinamidase/pyrazinamidase PncA is a member of a large family of hydrolase enzymes that catalyze the deamination of nicotinamide to nicotinic acid. PncA also functions as a pyrazinamidase in a wide variety of eubacteria and is an essential coenzyme in many cellular redox reactions in living systems. We report the crystal structure of substrate-free PncA from Bacillus subtilis (BsPncA) at 2.0 Å resolution to improve our understanding of the PncA family. The structure of BsPncA consists of an α/β domain and a subdomain. The subdomain of BsPncA has a different conformation than that of PncA enzymes from other organisms. The B-factor analysis revealed a rigid structure of the α/β domain, while the subdomain is highly flexible. Both dimers and tetramers were observed in BsPncA protein crystals, but only dimers were observed in solution. Our results provide useful information that will further enhance our understanding of the molecular functions of PncA family members.

PubMed: 30107912
DOI: 10.1016/j.bbrc.2018.08.067

実験手法

X-RAY DIFFRACTION (2 Å)