6A85

Crystal structure of a novel DNA quadruplex

Summary for 6A85

• Entry DOI: 10.2210/pdb6a85/pdb
• Descriptor: DNA (5'-D(*AP*GP*AP*GP*AP*GP*AP*TP*GP*GP*GP*TP*GP*CP*GP*TP*T)-3'), LITHIUM ION, SODIUM ION, ... (6 entities in total)
• Functional Keywords: dna, quadruplex
• Biological source: synthetic construct
• Total number of polymer chains: 2
• Total formula weight: 11186.95

Authors

Liu, H.H.,Gan, J.H. (deposition date: 2018-07-06, release date: 2019-03-06, Last modification date: 2024-03-27)

Primary citation

Liu, H.H.,Wang, R.,Yu, X.,Shen, F.S.,Lan, W.X.,Haruehanroengra, P.,Yao, Q.Q.,Zhang, J.,Chen, Y.Q.,Li, S.H.,Wu, B.X.,Zheng, L.N.,Ma, J.B.,Lin, J.Z.,Cao, C.Y.,Li, J.X.,Sheng, J.,Gan, J.H.
High-resolution DNA quadruplex structure containing all the A-, G-, C-, T-tetrads.
Nucleic Acids Res., 46:11627-11638, 2018

PubMed Abstract: DNA can form diverse structures, which predefine their physiological functions. Besides duplexes that carry the genetic information, quadruplexes are the most well-studied DNA structures. In addition to their important roles in recombination, replication, transcription and translation, DNA quadruplexes have also been applied as diagnostic aptamers and antidisease therapeutics. Herein we further expand the sequence and structure complexity of DNA quadruplex by presenting a high-resolution crystal structure of DNA1 (5'-AGAGAGATGGGTGCGTT-3'). This is the first quadruplex structure that contains all the internal A-, G-, C-, T-tetrads, A:T:A:T tetrads and bulged nucleotides in one single structure; as revealed by site-specific mutagenesis and biophysical studies, the central ATGGG motif plays important role in the quadruplex formation. Interestingly, our structure also provides great new insights into cation recognition, including the first-time reported Pb2+, by tetrad structures.

PubMed: 30285239
DOI: 10.1093/nar/gky902

Experimental method

X-RAY DIFFRACTION (1.45 Å)