6A85

Crystal structure of a novel DNA quadruplex

6A85 の概要

• エントリーDOI: 10.2210/pdb6a85/pdb
• 分子名称: DNA (5'-D(*AP*GP*AP*GP*AP*GP*AP*TP*GP*GP*GP*TP*GP*CP*GP*TP*T)-3'), LITHIUM ION, SODIUM ION, ... (6 entities in total)
• 機能のキーワード: dna, quadruplex
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 11186.95

構造登録者

Liu, H.H.,Gan, J.H. (登録日: 2018-07-06, 公開日: 2019-03-06, 最終更新日: 2024-03-27)

主引用文献

Liu, H.H.,Wang, R.,Yu, X.,Shen, F.S.,Lan, W.X.,Haruehanroengra, P.,Yao, Q.Q.,Zhang, J.,Chen, Y.Q.,Li, S.H.,Wu, B.X.,Zheng, L.N.,Ma, J.B.,Lin, J.Z.,Cao, C.Y.,Li, J.X.,Sheng, J.,Gan, J.H.
High-resolution DNA quadruplex structure containing all the A-, G-, C-, T-tetrads.
Nucleic Acids Res., 46:11627-11638, 2018

PubMed Abstract: DNA can form diverse structures, which predefine their physiological functions. Besides duplexes that carry the genetic information, quadruplexes are the most well-studied DNA structures. In addition to their important roles in recombination, replication, transcription and translation, DNA quadruplexes have also been applied as diagnostic aptamers and antidisease therapeutics. Herein we further expand the sequence and structure complexity of DNA quadruplex by presenting a high-resolution crystal structure of DNA1 (5'-AGAGAGATGGGTGCGTT-3'). This is the first quadruplex structure that contains all the internal A-, G-, C-, T-tetrads, A:T:A:T tetrads and bulged nucleotides in one single structure; as revealed by site-specific mutagenesis and biophysical studies, the central ATGGG motif plays important role in the quadruplex formation. Interestingly, our structure also provides great new insights into cation recognition, including the first-time reported Pb2+, by tetrad structures.

PubMed: 30285239
DOI: 10.1093/nar/gky902

実験手法

X-RAY DIFFRACTION (1.45 Å)