6A36

Mandelate oxidase mutant-Y128F with the 3-fluoropyruvic acid FMN adduct

Summary for 6A36

Entry DOI	10.2210/pdb6a36/pdb
Descriptor	4-hydroxymandelate oxidase, 1-{5-[(3S)-3-carboxy-4-fluoro-3-hydroxybutanoyl]-7,8-dimethyl-2,4-dioxo-1,3,4,5-tetrahydrobenzo[g]pteridin-10(2H)-yl}-1-deoxy-5-O-phosphono-D-ribitol, 1-deoxy-1-{5-[(1S)-2-fluoro-1-hydroxyethyl]-7,8-dimethyl-2,4-dioxo-1,3,4,5-tetrahydrobenzo[g]pteridin-10(2H)-yl}-5-O-phosphono-D-ribitol, ... (4 entities in total)
Functional Keywords	fmn-dependent oxidase, flavoprotein
Biological source	Amycolatopsis orientalis (Nocardia orientalis)
Total number of polymer chains	1
Total formula weight	41156.41
Authors	Li, T.L.,Lin, K.H. (deposition date: 2018-06-15, release date: 2019-06-19, Last modification date: 2023-11-22)
Primary citation	Lin, K.H.,Lyu, S.Y.,Yeh, H.W.,Li, Y.S.,Hsu, N.S.,Huang, C.M.,Wang, Y.L.,Shih, H.W.,Wang, Z.C.,Wu, C.J.,Li, T.L. Structural and chemical trapping of flavin-oxide intermediates reveals substrate-directed reaction multiplicity. Protein Sci., 29:1655-1666, 2020 Cited by PubMed: 32362037 DOI: 10.1002/pro.3879 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.44 Å)

Structure validation