6A2V

Crystal structure of Hcp protein

6A2V の概要

• エントリーDOI: 10.2210/pdb6a2v/pdb
• 分子名称: Type VI secretion system tube protein Hcp (2 entities in total)
• 機能のキーワード: hemolysin coregulated protein, type vi secretion system protein, structural protein
• 由来する生物種: Campylobacter jejuni subsp. jejuni
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 113762.89

構造登録者

Jobichen, C.,Sivaraman, J. (登録日: 2018-06-13, 公開日: 2018-09-19, 最終更新日: 2024-11-06)

主引用文献

Noreen, Z.,Jobichen, C.,Abbasi, R.,Seetharaman, J.,Sivaraman, J.,Bokhari, H.
Structural basis for the pathogenesis of Campylobacter jejuni Hcp1, a structural and effector protein of the Type VI Secretion System.
FEBS J., 285:4060-4070, 2018

PubMed Abstract: The Type VI Secretion System (T6SS) provides enhanced virulence to Campylobacter jejuni and has been associated with a high incidence of bloody diarrhea. The hemolysin-coregulated protein (Hcp)-the hallmark of the T6SS-can act as a structural and effector protein. Unlike other T6SS-harboring bacteria, which possess multiple Hcp proteins each performing different functions, C. jejuni possesses only one Hcp protein, and its structural and functional role has not been elucidated previously. Here, we report the structure and functional studies of Hcp from C. jejuni. We found similarities between the hexameric ring structure of Hcp-Cj and that of Hcp3 from Pseudomonas aeruginosa. Through functional studies, we showed two roles for Hcp-Cj that is, in cytotoxicity toward HepG2 cells and in biofilm formation in C. jejuni. In structure-based mutational analyses, we showed that an Arg-to-Ala mutation at position 30 within the extended loop results in a significant decrease in cytotoxicity, suggesting a role for this loop in binding to the host cell. However, this mutation does not affect its biofilm formation function. Collectively, this study supports the dual role of Hcp-Cj as a structural and effector protein in C. jejuni.

PubMed: 30194714
DOI: 10.1111/febs.14650

実験手法

X-RAY DIFFRACTION (2.588 Å)