6A12

X-ray structure of lipase from Geobacillus thermoleovorans

6A12 の概要

• エントリーDOI: 10.2210/pdb6a12/pdb
• 分子名称: Lipase, CALCIUM ION, ZINC ION, ... (4 entities in total)
• 機能のキーワード: lipase, geobacillus thermoleovorans, hydrolase
• 由来する生物種: Geobacillus thermoleovorans (Bacillus thermoleovorans)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 43433.80

構造登録者

Moharana, T.R.,Pal, B.,Rao, N.M. (登録日: 2018-06-06, 公開日: 2018-06-20, 最終更新日: 2023-11-22)

主引用文献

Moharana, T.R.,Pal, B.,Rao, N.M.
X-ray structure and characterization of a thermostable lipase from Geobacillus thermoleovorans.
Biochem. Biophys. Res. Commun., 508:145-151, 2019

PubMed Abstract: Thermo-alkalophilic bacterium, Geobacillus thermoleovorans secrets many enzymes including a 43 kDa extracellular lipase. Significant thermostability, organic solvent stability and wide substrate preferences for hydrolysis drew our attention to solve its structure by crystallography. The structure was solved by molecular replacement method and refined up to 2.14 Å resolution. Structure of the lipase showed an alpha-beta fold with 19 α-helices and 10 β-sheets. The active site remains covered by a lid. One calcium and one zinc atom was found in the crystal. The structure showed a major difference (rmsd 5.6 Å) from its closest homolog in the amino acid region 191 to 203. Thermal unfolding of the lipase showed that the lipase is highly stable with T of 76 °C. C NMR spectra of products upon triglyceride hydrolysate revealed that the lipase hydrolyses at both sn-1 and sn-2 positions with equal efficiency.

PubMed: 30471860
DOI: 10.1016/j.bbrc.2018.11.105

実験手法

X-RAY DIFFRACTION (2.145 Å)