5ZYQ

The Structure of Human PAF1/CTR9 complex

5ZYQ の概要

• エントリーDOI: 10.2210/pdb5zyq/pdb
• 分子名称: RNA polymerase-associated protein CTR9 homolog,RNA polymerase II-associated factor 1 homolog (2 entities in total)
• 機能のキーワード: transcription, complex, tpr
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36374.41

構造登録者

Xie, Y.,Zheng, M.,Zhou, H.,Long, J. (登録日: 2018-05-28, 公開日: 2018-09-26, 最終更新日: 2024-03-27)

主引用文献

Xie, Y.,Zheng, M.,Chu, X.,Chen, Y.,Xu, H.,Wang, J.,Zhou, H.,Long, J.
Paf1 and Ctr9 subcomplex formation is essential for Paf1 complex assembly and functional regulation.
Nat Commun, 9:3795-3795, 2018

PubMed Abstract: The evolutionarily conserved multifunctional polymerase-associated factor 1 (Paf1) complex (Paf1C), which is composed of at least five subunits (Paf1, Leo1, Ctr9, Cdc73, and Rtf1), plays vital roles in gene regulation and has connections to development and human diseases. Here, we report two structures of each of the human and yeast Ctr9/Paf1 subcomplexes, which assemble into heterodimers with very similar conformations, revealing an interface between the tetratricopeptide repeat module in Ctr9 and Paf1. The structure of the Ctr9/Paf1 subcomplex may provide mechanistic explanations for disease-associated mutations in human PAF1 and CTR9. Our study reveals that the formation of the Ctr9/Paf1 heterodimer is required for the assembly of yeast Paf1C, and is essential for yeast viability. In addition, disruption of the interaction between Paf1 and Ctr9 greatly affects the level of histone H3 methylation in vivo. Collectively, our results shed light on Paf1C assembly and functional regulation.

PubMed: 30228257
DOI: 10.1038/s41467-018-06237-7

実験手法

X-RAY DIFFRACTION (2.531 Å)