5ZWO

Cryo-EM structure of the yeast B complex at average resolution of 3.9 angstrom

5ZWO の概要

• エントリーDOI: 10.2210/pdb5zwo/pdb
• 関連するPDBエントリー: 5ZWM 5ZWN
• EMDBエントリー: 6972 6973 6974
• 分子名称: Pre-mRNA-splicing factor 8, U6 snRNA-associated Sm-like protein LSm2, U6 snRNA-associated Sm-like protein LSm3, ... (48 entities in total)
• 機能のキーワード: spliceosome, assembly, b complex, splicing
• 由来する生物種: Saccharomyces cerevisiae S288c (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 60
• 化学式量合計: 2604025.39

構造登録者

Bai, R.,Wan, R.,Yan, C.,Shi, Y. (登録日: 2018-05-16, 公開日: 2018-08-29, 最終更新日: 2024-03-27)

主引用文献

Bai, R.,Wan, R.,Yan, C.,Lei, J.,Shi, Y.
Structures of the fully assembledSaccharomyces cerevisiaespliceosome before activation
Science, 360:1423-1429, 2018

PubMed Abstract: The precatalytic spliceosome (B complex) is preceded by the pre-B complex. Here we report the cryo-electron microscopy structures of the pre-B and B complexes at average resolutions of 3.3 to 4.6 and 3.9 angstroms, respectively. In the pre-B complex, the duplex between the 5' splice site (5'SS) and U1 small nuclear RNA (snRNA) is recognized by Yhc1, Luc7, and the Sm ring. In the B complex, U1 small nuclear ribonucleoprotein is dissociated, the 5'-exon-5'SS sequences are translocated near U6 snRNA, and three B-specific proteins may orient the precursor messenger RNA. In both complexes, U6 snRNA is anchored to loop I of U5 snRNA, and the duplex between the branch point sequence and U2 snRNA is recognized by the SF3b complex. Structural analysis reveals the mechanism of assembly and activation for the yeast spliceosome.

PubMed: 29794219
DOI: 10.1126/science.aau0325

実験手法

ELECTRON MICROSCOPY (3.9 Å)