5ZUH

Solution structure of RRM domain of La protein from Trypanosoma brucei

「5ZOM」から置き換えられました

5ZUH の概要

• エントリーDOI: 10.2210/pdb5zuh/pdb
• NMR情報: BMRB: 36185
• 分子名称: RNA binding protein La-like protein (1 entity in total)
• 機能のキーワード: la motif, rrm domain, rna-binding, trypanosoma brucei, rna binding protein
• 由来する生物種: Trypanosoma brucei
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11282.73

構造登録者

Shan, F.Z. (登録日: 2018-05-07, 公開日: 2019-05-15, 最終更新日: 2024-05-01)

主引用文献

Shan, F.,Mei, S.,Zhang, J.,Zhang, X.,Xu, C.,Liao, S.,Tu, X.
A telomerase subunit homolog La protein from Trypanosoma brucei plays an essential role in ribosomal biogenesis.
Febs J., 286:3129-3147, 2019

PubMed Abstract: The autoantigen La protein is an important component of telomerase and a predominantly nuclear phosphoprotein. As a telomerase subunit, La protein associates with the telomerase ribonucleoprotein and influences telomere length. In the reverse transcription, La protein stimulates enzymatic activity and increases repeated addition processivity of telomerase. As nuclear phosphoprotein, La protein binds the 3' poly(U)-rich elements of nascent RNA polymerase III transcripts to facilitate its correct folding and maturation. In this work, we identified a La protein homolog (TbLa) from Trypanosoma brucei (T. brucei). We revealed that TbLa interacts with ribosome-associated protein P34/P37, 40S ribosomal protein SA, and 60S ribosomal subunit L5 in T. brucei. In the interactions between TbLa protein and (P34/P37)/L5/SA, RNA recognition motif (RRM) domain of TbLa was indicated to make the major contribution to the processes. We determined the solution structure of TbLa RRM domain. NMR chemical shift perturbations revealed that the positively charged RNA-binding pocket of TbLa RRM domain is responsible for its interaction with ribosomal and ribosome-associated proteins P37/L5/SA. Furthermore, depletion of TbLa affected the maturation process of 5S rRNA and ribosomal assembly, suggesting TbLa protein might play a significant role in the ribosomal biogenesis pathway in T. brucei. Taken together, our results provide a novel insight and structural basis for better understanding the roles of TbLa and RRM domain in ribosomal biogenesis in T. brucei. DATABASE: Structural data are available in the PDB under the accession number 5ZUH.

PubMed: 30993866
DOI: 10.1111/febs.14853

実験手法

SOLUTION NMR