5ZR6

Manganese-dependent transcriptional repressor complex with manganese

5ZR6 の概要

• エントリーDOI: 10.2210/pdb5zr6/pdb
• 分子名称: Metal-dependent transcriptional regulator, MANGANESE (II) ION (3 entities in total)
• 機能のキーワード: transcriptional repressor, transcription
• 由来する生物種: Mycobacterium tuberculosis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 50294.01

構造登録者

Cong, X.Y.,Xu, S.J.,Wang, J.B. (登録日: 2018-04-23, 公開日: 2019-03-20, 最終更新日: 2024-03-27)

主引用文献

Cong, X.Y.,Yuan, Z.L.,Wang, Z.,Wei, B.,Xu, S.J.,Wang, J.B.
Crystal structures of manganese-dependent transcriptional repressor MntR (Rv2788) from Mycobacterium tuberculosis in apo and manganese bound forms.
Biochem. Biophys. Res. Commun., 501:423-427, 2018

PubMed Abstract: The pathogenic Mycobacterium tuberculosis encodes two members of the DtxR family metalloregulators, IdeR and MntR. IdeR represses gene expression in response to ferrous iron, while MntR (Rv2788) functions as a manganese-dependent transcriptional repressor, which represses the expression of manganese transporter genes to maintain manganese homeostasis. Although the structural study towards IdeR is in-depth, there is no MntR structure available. Herein, we report both apo and manganese bound forms of MntR structures from M. tuberculosis. MntR has evolved into two metal ion binding sites like other DtxR proteins and for the first time, we captured the two sites fully occupied by its natural ions with one Mn ion at the first site and two Mn ions at the second binding site (binuclear manganese cluster). The conformation change of MntR resulting from manganese binding could prime the MntR for DNA binding, which is a conserved activation mechanism among DtxR family.

PubMed: 29730293
DOI: 10.1016/j.bbrc.2018.05.005

実験手法

X-RAY DIFFRACTION (3 Å)