5ZOR
Solution structure of centrin4 from Trypanosoma brucei
Summary for 5ZOR
| Entry DOI | 10.2210/pdb5zor/pdb |
| NMR Information | BMRB: 36179 |
| Descriptor | Centrin, putative (1 entity in total) |
| Functional Keywords | centrin, ef-hand, calcium binding, trypanosoma brucei, metal binding protein |
| Biological source | Trypanosoma brucei brucei (strain 927/4 GUTat10.1) |
| Total number of polymer chains | 1 |
| Total formula weight | 16570.63 |
| Authors | Shan, F.Z.,Tu, X.M. (deposition date: 2018-04-15, release date: 2019-03-13, Last modification date: 2024-05-01) |
| Primary citation | Shan, F.Z.,Ye, K.Q.,Zhang, J.H.,Liao, S.H.,Zhang, X.C.,Xu, C.,Tu, X.M. Solution structure of TbCentrin4 fromTrypanosoma bruceiand its interactions with Ca2+and other centrins. Biochem. J., 475:3763-3778, 2018 Cited by PubMed Abstract: Centrin is a conserved calcium-binding protein that plays an important role in diverse cellular biological processes such as ciliogenesis, gene expression, DNA repair and signal transduction. In , TbCentrin4 is mainly localized in basal bodies and bi-lobe structure, and is involved in the processes coordinating karyokinesis and cytokinesis. In the present study, we solved the solution structure of TbCentrin4 using NMR (nuclear magnetic resonance) spectroscopy. TbCentrin4 contains four EF-hand motifs consisting of eight α-helices. Isothermal titration calorimetry experiment showed that TbCentrin4 has a strong Ca binding ability. NMR chemical shift perturbation indicated that TbCentrin4 binds to Ca through its C-terminal domain composed of EF-hand 3 and 4. Meanwhile, we revealed that TbCentrin4 undergoes a conformational change and self-assembly induced by high concentration of Ca Intriguingly, localization of TbCentrin4 was dispersed or disappeared from basal bodies and the bi-lobe structure when the cells were treated with Ca, implying the influence of Ca on the cellular functions of TbCentrin4. Besides, we observed the interactions between TbCentrin4 and other Tbcentrins and revealed that the interactions are Ca dependent. Our findings provide a structural basis for better understanding the biological functions of TbCentrin4 in the relevant cellular processes. PubMed: 30389845DOI: 10.1042/BCJ20180752 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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