5ZG9

Crystal structure of MoSub1-ssDNA complex in phosphate buffer

5ZG9 の概要

• エントリーDOI: 10.2210/pdb5zg9/pdb
• 分子名称: MoSub1, DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*G)-3'), PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: ssdna binding protein, transcriptional co-factor, pc4-like protein, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Magnaporthe oryzae (strain P131) (Rice blast fungus); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 42850.50

構造登録者

Zhao, Y.,Huang, J.,Liu, H.,Yi, L.,Wang, S.,Zhang, X.,Liu, J. (登録日: 2018-03-08, 公開日: 2019-03-27, 最終更新日: 2024-03-27)

主引用文献

Zhao, Y.,Zhang, Y.,Huang, J.,Wang, S.,Yi, L.,Zhang, X.,Xu, M.,Fang, X.,Liu, J.
The effect of phosphate ion on the ssDNA binding mode of MoSub1, a Sub1/PC4 homolog from rice blast fungus.
Proteins, 87:257-264, 2019

PubMed Abstract: MoSub1 is an ortholog of yeast single stranded DNA binding protein Sub1 or human PC4 from rice blast fungus. All of them share a similar DNA binding region and may have similar biological roles. The well-studied Sub1/PC4 has been reported to play multiple roles in DNA metabolic processes, such as transcription and DNA repair and their DNA binding capacity is significantly affected by phosphorylation. Here, we determined the crystal structure of MoSub1 complexed with ssDNA in a phosphate solution. The crystal structure of the MoSub1-ssDNA complex was solved to a resolution of 2.04 Å. A phosphate ion at the interface of the protein-DNA interaction of the complex bridged the lys84 of the protein and two nucleotides. The DNA was bound in novel mode (L mode) in the MoSub1 complex in the presence of phosphate ions, while DNA bound in the straight mode in the absence of the phosphate ion and in U mode in the same binding motif of the PC4-ssDNA complex. The crystal structure of the complex and a small-angle X-ray scattering analysis revealed that the phosphate ion at the protein-DNA interface affected the DNA binding mode of MoSub1 to oligo-DNA and provided a new structural clue for studying its functions.

PubMed: 30561148
DOI: 10.1002/prot.25647

実験手法

X-RAY DIFFRACTION (2.04 Å)