5ZFO

NMR structure of IRD12 from Capsicum annum.

5ZFO の概要

• エントリーDOI: 10.2210/pdb5zfo/pdb
• NMR情報: BMRB: 36171
• 分子名称: Pin-II type proteinase inhibitor 38 (1 entity in total)
• 機能のキーワード: protein, inhibitor, capsicum annum, plant protein
• 由来する生物種: Capsicum annuum (Bell pepper)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5429.05

構造登録者

Gartia, J.,Barnwal, R.P.,Chary, K.V.R. (登録日: 2018-03-06, 公開日: 2019-05-15, 最終更新日: 2024-11-06)

主引用文献

Gartia, J.,Anangi, R.,Joshi, R.S.,Giri, A.P.,King, G.F.,Barnwal, R.P.,Chary, K.V.R.
NMR structure and dynamics of inhibitory repeat domain variant 12, a plant protease inhibitor from Capsicum annuum, and its structural relationship to other plant protease inhibitors.
J.Biomol.Struct.Dyn., :1-10, 2019

PubMed Abstract: Although several plant protease inhibitors have been structurally characterized using X-ray crystallography, very few have been studied using NMR techniques. Here, we report an NMR study of the solution structure and dynamics of an inhibitory repeat domain (IRD) variant 12 from the wound-inducible Pin-II type proteinase inhibitor from . IRD variant 12 (IRD12) showed strong anti-metabolic activity against the Lepidopteran insect pest, . The NMR-derived three-dimensional structure of IRD12 reveals a three-stranded anti-parallel β-sheet rigidly held together by four disulfide bridges and shows structural homology with known IRDs. It is interesting to note that the IRD12 structure containing ∼75% unstructured part still shows substantial amount of rigidity of N-H bond vectors with respect to its molecular motion.Communicated by Ramaswamy H. Sarma.

PubMed: 31038412
DOI: 10.1080/07391102.2019.1607559

実験手法

SOLUTION NMR