5ZCY

Crystal structure of archaeal translation initiation factor 1 at 1.5 Angstroms resolution

5ZCY の概要

• エントリーDOI: 10.2210/pdb5zcy/pdb
• 分子名称: Protein translation factor SUI1 homolog, NITRATE ION, NITRITE ION, ... (4 entities in total)
• 機能のキーワード: aif1, eif1, eukaryotic translation initiation, ycih, archaea, eukaryote, translation
• 由来する生物種: Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11582.45

構造登録者

Gogoi, P.,Kanaujia, S.P. (登録日: 2018-02-22, 公開日: 2018-05-02, 最終更新日: 2023-11-22)

主引用文献

Gogoi, P.,Kanaujia, S.P.
Archaeal and eukaryal translation initiation factor 1 differ in their RNA interacting loops.
FEBS Lett., 592:1602-1610, 2018

PubMed Abstract: The archaeal translation initiation factor 1 (aIF1) is reported to be functionally homologous to the eukaryotic translation initiation factor 1 (eIF1). However, lack of a structural comparison between aIF1 and eIF1 has limited our understanding of the structural (dis)similarities. Herein, we have determined the three-dimensional crystal structure of an open reading frame PH1771.1 encoding aIF1 in Pyrococcus horikoshii OT3. Results reveal that although aIF1 has low sequence similarity with eIF1, high structural homology exists between the two proteins. Nonetheless, notable critical differences between aIF1 and eIF1 could still be perceived at the β -β basic loop, the acidic loop and the solvent-exposed surface. These differences might lead to a slightly divergent mode of action of aIF1 during archaeal translation initiation.

PubMed: 29608219
DOI: 10.1002/1873-3468.13044

実験手法

X-RAY DIFFRACTION (1.5 Å)