5ZBS

Crystal Structure of Kinesin-3 KIF13B motor Y73C mutant

Summary for 5ZBS

• Entry DOI: 10.2210/pdb5zbs/pdb
• Descriptor: Kinesin family member 13B, MAGNESIUM ION, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... (4 entities in total)
• Functional Keywords: kinesin, atpase, transport protein
• Biological source: Rattus norvegicus (Rat)
• Total number of polymer chains: 3
• Total formula weight: 126187.81

Authors

Ren, J.Q.,Wang, S.,Feng, W. (deposition date: 2018-02-12, release date: 2018-05-30, Last modification date: 2023-11-22)

Primary citation

Ren, J.,Zhang, Y.,Wang, S.,Huo, L.,Lou, J.,Feng, W.
Structural Delineation of the Neck Linker of Kinesin-3 for Processive Movement.
J. Mol. Biol., 430:2030-2041, 2018

PubMed Abstract: Processive kinesin motors contain a neck linker (NL) that mediates the chemo-mechanical coupling and controls the directionality and processivity. However, kinesin-3 NL remains poorly determined due to the lack of the structural information of the junction with the following neck coil (NC). Here, we determined the structure of the motor domain (MD)-NL-NC tandem of KIF13B that defines the junction between NL and NC and delineates kinesin-3 NL. Unexpectedly, the length of kinesin-3 NL is much shorter than the previously predicted one. In the MD-NL-NC structure, NL docks onto the MD with a conventional mode but the interaction between NL and the MD is relatively weak due to the shorter N-terminal cover strand of the MD. The optimal short NL and its weak interaction with the MD would generate the tight inter-head strain and facilitate the NL undocking, which may contribute to the fast and superprocessive motility of kinesin-3.

PubMed: 29752968
DOI: 10.1016/j.jmb.2018.05.010

Experimental method

X-RAY DIFFRACTION (2.203 Å)