5Z7X

Crystal structure of Striga hermonthica HTL4 (ShHTL4)

5Z7X の概要

• エントリーDOI: 10.2210/pdb5z7x/pdb
• 分子名称: Hyposensitive to light 4, 1,2-ETHANEDIOL, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: hydrolase activity, putative receptor of strigolactone, hydrolase
• 由来する生物種: Striga hermonthica (Purple witchweed)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30278.91

構造登録者

Xu, Y.,Miyakawa, T.,Nakamura, A.,Tanokura, M. (登録日: 2018-01-30, 公開日: 2018-08-29, 最終更新日: 2023-11-22)

主引用文献

Xu, Y.,Miyakawa, T.,Nosaki, S.,Nakamura, A.,Lyu, Y.,Nakamura, H.,Ohto, U.,Ishida, H.,Shimizu, T.,Asami, T.,Tanokura, M.
Structural analysis of HTL and D14 proteins reveals the basis for ligand selectivity in Striga.
Nat Commun, 9:3947-3947, 2018

PubMed Abstract: HYPOSENSITIVE TO LIGHT (HTL) and DWARF14 (D14) mediate the perception of karrikin and strigolactone, which stimulates germination of the parasitic weed Striga. However, their role in parasitic seeds is poorly understood, and the basis for their differing responsiveness remains unclear. Here, we show that Striga hermonthica HTL proteins (ShHTLs) in 'conserved' and 'intermediate' clades are able to bind karrikin. The 'divergent' clade is able to hydrolyze strigolactone. Unexpectedly, we find that ShD14 is also capable of hydrolyzing strigolactone. Through comparative analysis of ShHTLs and ShD14 crystal structures, we provide insights into the basis for their selectivity. Moreover, we show that both ShD14 and divergent clade ShHTLs, but not conserved and intermediate clade ShHTLs, can interact with the putative downstream signaling component ShMAX2 in the presence of the synthetic strigolactone, rac-GR24. These findings provide insight into how strigolactone is perceived and how ligand specificity is determined.

PubMed: 30258184
DOI: 10.1038/s41467-018-06452-2

実験手法

X-RAY DIFFRACTION (2.055 Å)