5Z7M

SmChiA sliding-intermediate with chitohexaose

5Z7M の概要

• エントリーDOI: 10.2210/pdb5z7m/pdb
• 分子名称: Chitinase A, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: chitinase, complex, intermediate, hydrolase
• 由来する生物種: Serratia marcescens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 62298.34

構造登録者

Nakamura, A.,Iino, R. (登録日: 2018-01-30, 公開日: 2018-09-26, 最終更新日: 2024-11-13)

主引用文献

Nakamura, A.,Okazaki, K.I.,Furuta, T.,Sakurai, M.,Iino, R.
Processive chitinase is Brownian monorail operated by fast catalysis after peeling rail from crystalline chitin.
Nat Commun, 9:3814-3814, 2018

PubMed Abstract: Processive chitinase is a linear molecular motor which moves on the surface of crystalline chitin driven by processive hydrolysis of single chitin chain. Here, we analyse the mechanism underlying unidirectional movement of Serratia marcescens chitinase A (SmChiA) using high-precision single-molecule imaging, X-ray crystallography, and all-atom molecular dynamics simulation. SmChiA shows fast unidirectional movement of ~50 nm s with 1 nm forward and backward steps, consistent with the length of reaction product chitobiose. Analysis of the kinetic isotope effect reveals fast substrate-assisted catalysis with time constant of ~3 ms. Decrystallization of the single chitin chain from crystal surface is the rate-limiting step of movement with time constant of ~17 ms, achieved by binding free energy at the product-binding site of SmChiA. Our results demonstrate that SmChiA operates as a burnt-bridge Brownian ratchet wherein the Brownian motion along the single chitin chain is rectified forward by substrate-assisted catalysis.

PubMed: 30232340
DOI: 10.1038/s41467-018-06362-3

実験手法

X-RAY DIFFRACTION (2 Å)