5Z6Q

Crystal structure of AAA of Spastin

5Z6Q の概要

• エントリーDOI: 10.2210/pdb5z6q/pdb
• 分子名称: Spastin, CHLORIDE ION (2 entities in total)
• 機能のキーワード: spastin, hydrolase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42780.28

構造登録者

Lin, Z.,Wang, C.,Shen, Y. (登録日: 2018-01-25, 公開日: 2018-12-05, 最終更新日: 2024-03-27)

主引用文献

Fan, X.,Lin, Z.,Fan, G.,Lu, J.,Hou, Y.,Habai, G.,Sun, L.,Yu, P.,Shen, Y.,Wen, M.,Wang, C.
The AAA protein spastin possesses two levels of basal ATPase activity
FEBS Lett., 592:1625-1633, 2018

PubMed Abstract: The AAA ATPase spastin is a microtubule-severing enzyme that plays important roles in various cellular events including axon regeneration. Herein, we found that the basal ATPase activity of spastin is negatively regulated by spastin concentration. By determining a spastin crystal structure, we demonstrate the necessity of intersubunit interactions between spastin AAA domains. Neutralization of the positive charges in the microtubule-binding domain (MTBD) of spastin dramatically decreases the ATPase activity at low concentration, although the ATP-hydrolyzing potential is not affected. These results demonstrate that, in addition to the AAA domain, the MTBD region of spastin is also involved in regulating ATPase activity, making interactions between spastin protomers more complicated than expected.

PubMed: 29710391
DOI: 10.1002/1873-3468.13075

実験手法

X-RAY DIFFRACTION (3 Å)