5Z5Q

Nukacin ISK-1 in active state

5Z5Q の概要

• エントリーDOI: 10.2210/pdb5z5q/pdb
• NMR情報: BMRB: 36157
• 分子名称: Lantibiotic nukacin (1 entity in total)
• 機能のキーワード: lantibiotics, antibiotic
• 由来する生物種: Staphylococcus warneri
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2970.53

構造登録者

Kohda, D.,Fujinami, D. (登録日: 2018-01-19, 公開日: 2018-11-28, 最終更新日: 2024-07-10)

主引用文献

Fujinami, D.,Mahin, A.A.,Elsayed, K.M.,Islam, M.R.,Nagao, J.I.,Roy, U.,Momin, S.,Zendo, T.,Kohda, D.,Sonomoto, K.
The lantibiotic nukacin ISK-1 exists in an equilibrium between active and inactive lipid-II binding states.
Commun Biol, 1:150-150, 2018

PubMed Abstract: The lantibiotic nukacin ISK-1 exerts antimicrobial activity through binding to lipid II. Here, we perform NMR analyses of the structure of nukacin ISK-1 and the interaction with lipid II. Unexpectedly, nukacin ISK-1 exists in two structural states in aqueous solution, with an interconversion rate on a time scale of seconds. The two structures differ in the relative orientations of the two lanthionine rings, ring A and ring C. Chemical shift perturbation induced by the titration of lipid II reveals that only one state was capable of binding to lipid II. On the molecular surface of the active state, a multiple hydrogen-bonding site formed by amino acid residues in the ring A region is adjacent to a hydrophobic surface formed by residues in the ring C region, and we propose that these sites interact with the pyrophosphate moiety and the isoprene chain of the lipid II molecule, respectively.

PubMed: 30272026
DOI: 10.1038/s42003-018-0150-3

実験手法

SOLUTION NMR