5Z1F

Structure of atOSCA3.1 channel

5Z1F の概要

• エントリーDOI: 10.2210/pdb5z1f/pdb
• EMDBエントリー: 6875
• 分子名称: CSC1-like protein ERD4 (1 entity in total)
• 機能のキーワード: osca, tmem63, ion channel, mechanosensitive, membrane protein, osmosensing, metal transport
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 164035.45

構造登録者

Chen, L.,Zhang, M.,Kang, Y.,Wu, J.X. (登録日: 2017-12-26, 公開日: 2018-09-12, 最終更新日: 2024-03-27)

主引用文献

Zhang, M.,Wang, D.,Kang, Y.,Wu, J.X.,Yao, F.,Pan, C.,Yan, Z.,Song, C.,Chen, L.
Structure of the mechanosensitive OSCA channels.
Nat. Struct. Mol. Biol., 25:850-858, 2018

PubMed Abstract: Mechanosensitive ion channels convert mechanical stimuli into a flow of ions. These channels are widely distributed from bacteria to higher plants and humans, and are involved in many crucial physiological processes. Here we show that two members of the OSCA protein family in Arabidopsis thaliana, namely AtOSCA1.1 and AtOSCA3.1, belong to a new class of mechanosensitive ion channels. We solve the structure of the AtOSCA1.1 channel at 3.5-Å resolution and AtOSCA3.1 at 4.8-Å resolution by cryo-electron microscopy. OSCA channels are symmetric dimers that are mediated by cytosolic inter-subunit interactions. Strikingly, they have structural similarity to the mammalian TMEM16 family proteins. Our structural analysis accompanied with electrophysiological studies identifies the ion permeation pathway within each subunit and suggests a conformational change model for activation.

PubMed: 30190597
DOI: 10.1038/s41594-018-0117-6

実験手法

ELECTRON MICROSCOPY (4.8 Å)